Project information
Analýza klonálních progenitorů plazmatických buněk u monoklonálních gamapatií
- Project Identification
- GAP304/10/1395
- Project Period
- 1/2010 - 12/2014
- Investor / Pogramme / Project type
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Czech Science Foundation
- Standard Projects
- MU Faculty or unit
- Faculty of Medicine
- Keywords
- Monoclonal gammopathies; plasma cells; progenitors; phenotype; flow cytometry
Detailed study of heterogeneous subpopulations of myeloma cells and their precursors/progenitors in monoclonal gammopathy patients can lead to identification and characterisation of myeloma stem cells. Using polychromatic flow cytometry with sorting of cells, phenotype of myeloma stem cells can be detected and different subpopulation can be selected for clonality assessment. In vitro culturing then can prove self renewal capacity of these myeloma stem cells and their multidrug resistance as well. We suppose that using these approaches we are able to obtain new information in area of pathophysiology of myeloma cells and their behaviour, which can be potentially used for determination of predictive factors applicable in progression of monoclonal gammopaty of undetermined significance to multiple myeloma.
Publications
Total number of publications: 108
2011
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Correlation of plasma cell phenotype with cytogenetic and morphological findings in multiple myeloma
Year: 2011, type: Conference abstract
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Flow cytometric analysis of CD4 and CD8 regulatory cells before and after lenalidomide plus dexamethasone treatment in multiple myeloma patients
Year: 2011, type: Conference abstract
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Flow Cytometric Phenotyping and Analysis of T Regulatory Cells in Multiple Myeloma Patients
Klinická onkologie, year: 2011, volume: 24, edition: Suppl.
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Flow Cytometry in Monoclonal Gammopathies
Klinická onkologie, year: 2011, volume: 24, edition: Suppl.
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Genomics in Multiple Myeloma Research
Klinická onkologie, year: 2011, volume: 24, edition: Suppl.
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Chromosomal abnormalities in monoclonal gammopathy of undetermined significance patients analyzed by fluorescence in situ hybridization and oligonucleotide based array comparative genomic hybridization
Year: 2011, type: Conference abstract
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Impact of Nestin Analysis in Multiple Myeloma
Klinická onkologie, year: 2011, volume: 24, edition: Suppl.
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Induction by lenalidomide and dexamethasone combination increase CD4 and CD8 regulatory cells of newly diagnosed multiple myeloma patients
Year: 2011, type: Conference abstract
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Induction by lenalidomide plus dexamethasone elevates regulatory and total lymphocyte populations in newly diagnosed multiple myeloma patients: A single center study
Year: 2011, type: Conference abstract
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Levels of nestin protein correlate with 1q21 gain and hyperdiploidy in multiple myeloma
Year: 2011, type: Conference abstract