Project information
Comprehensive prognostic and predictive panel for chronic lymphocytic leukemia: a next-generation sequencing tool suitable for clinical practice and study of genetic architecture behind the disease progress

Information

This project doesn't include Faculty of Science. It includes Central European Institute of Technology. Official project website can be found on muni.cz.

Project Identification

NV19-03-00091

Project Period

5/2019 - 12/2022

Investor / Pogramme / Project type

Ministry of Health of the CR

Ministry of Health Research Programme 2015 - 2022

MU Faculty or unit

Central European Institute of Technology

prof. RNDr. Šárka Pospíšilová, Ph.D.

Cooperating Organization

University Hospital Brno-Bohunice

Responsible person Mgr. Veronika Navrkalová, Ph.D.

Chronic lymphocytic leukemia (CLL) is common and still incurable leukemia of adults characterized by remarkable genetic and clinical heterogeneity. In proposed project we aim to study genetic background underlying clonal evolution of genomic alterations in treatment naïve CLL patients taking the advantage of availability of consecutive samples collected during disease course. Our main objective is to early disclose the disease progression and therapy need. We plan to assess both the prognostic and the predictive value of genomic aberrations detected just before treatment start. For this purpose, a highly complex next-generation sequencing (NGS) tool analyzing simultaneously both fundamental, as well as novel CLL biomarkers will be implemented. We employ benefits of capture-based NGS technology enabling to analyze in one test IGHV mutational status, recurrent chromosomal and gene defects, and Ig light chains clonality. We presume that our NGS panel tailored for CLL can be widely adopted in clinical practice as a simple comprehensive test for better patient prognostication.

Publications

Total number of publications: 45

2021

LYNX (Lymphoid next-generation sequencing): NGS panel a bioinformatický nástroj pro integrativní analýzu prognostických a prediktivních markerů B-buněčných lymfoproliferací.

NAVRKALOVÁ Veronika PLEVOVÁ Karla REIGL Tomáš HYNŠT Jakub PÁL Karol PORC Jakub Paweł MAREČKOVÁ Andrea JELÍNKOVÁ Hana JANÍKOVÁ Andrea DOUBEK Michael POSPÍŠILOVÁ Šárka

Conference abstract

Year: 2021, type: Conference abstract
LYNX: an interactive bioinformatic tool for capture-based next-generation sequencing data analysis in lymphoid malignancies.

REIGL Tomáš NAVRKALOVÁ Veronika HYNŠT Jakub PORC Jakub Paweł PÁL Karol HRON Tomáš DARZENTAS Nikos POSPÍŠILOVÁ Šárka HALBHUBER Z. PLEVOVÁ Karla

Conference abstract

Year: 2021, type: Conference abstract
Maskovaná hypodiploidie u pacientů s akutní lymfoblastickou leukémií

BRYJOVÁ Lenka BRHELOVÁ Eva ARPÁŠ Tomáš ONDROUŠKOVÁ Eva ŠEJNOHOVÁ Markéta JELÍNKOVÁ Hana HRABOVSKÝ Štěpán ORŠULOVÁ Martina VRZALOVÁ Zuzana BULIKOVÁ Alena NAVRKALOVÁ Veronika FOLBER František ŠEDOVÁ Petra MAYER Jiří POSPÍŠILOVÁ Šárka DOUBEK Michael JAROŠOVÁ Marie

Conference abstract

Year: 2021, type: Conference abstract
RPS15 mutations rewire RNA translation in chronic lymphocytic leukemia

NTOUFA S. GEROUSI M. LAIDOU S. PSOMOPOULOS F. TSIOLAS G. MOYSIADIS T. PAPAKONSTANTINOU N. MANSOURI L. ANAGNOSTOPOULOS A. STAVROGIANNI N. POSPÍŠILOVÁ Šárka PLEVOVÁ Karla MAKRIS A.M. ROSENQUIST R. STAMATOPOULOS K.

Article in Periodical

BLOOD ADVANCES, year: 2021, volume: 5, edition: 13, DOI
T-buněčný lymfoblastický lymfom (T-LBL) dětského věku s kazuistikou geneticky zajímavého případu

JAROŠOVÁ Marie ONDROUŠKOVÁ Eva BRZOBOHATÁ Anna ŠMEJKAL Jiří DOKOUPILOVÁ Lenka MEJSTŘÍKOVÁ Soňa OLTOVÁ Alexandra JEŽOVÁ Marta NAVRKALOVÁ Veronika KOTAŠKOVÁ Jana ŠURKOVÁ Silvie KŘENOVÁ Zdenka PÁLOVÁ Hana VESELÁ Petra KEPÁK Tomáš MUDRÝ Petr ŠTĚRBA Jaroslav

Conference abstract

Year: 2021, type: Conference abstract

2020

Capture-based NGS panel for detection of immunoglobulin and T-cell receptor gene rearrangements in DNA samples from patients with acute lymphoblastic leukemia

JELÍNKOVÁ Hana NAVRKALOVÁ Veronika PLEVOVÁ Karla PORC Jakub Paweł HYNŠT Jakub PÁL Karol REIGL Tomáš DARZENTAS Nikos DOUBEK Michael ŽENATOVÁ Marcela MAREČKOVÁ Andrea POSPÍŠILOVÁ Šárka

Conference abstract

Year: 2020, type: Conference abstract
International prognostic score for asymptomatic early-stage chronic lymphocytic leukemia

CONDOLUCI A. DI BERGAMO L. T. LANGERBEINS P. HOECHSTETTER M. A. HERLING C. D. DE PAOLI L. DELGADO J. RABE K. G. GENTILE M. DOUBEK Michael MAURO F. R. CHIODIN G. MATTSSON M. BAHLO J. CUTRONA G. KOTAŠKOVÁ Jana DEAMBROGI C. SMEDBY K. E. SPINA V. BRUSCAGGIN A. WU W. MOIA R. BIANCHI E. GERBER B. ZUCCA E. GILLESSEN S. GHIELMINI M. CAVALLI F. STUSSI G. HESS M. A. BAUMANN T. S. NERI A. FERRARINI M. ROSENQUIST R. FORCONI F. FOA R. POSPÍŠILOVÁ Šárka MORABITO F. STILGENBAUER S. DOHNER H. PARIKH S. A. WIERDA W. G. MONTSERRAT E. GAIDANO G. HALLEK M. ROSSI D.

Article in Periodical

Blood, year: 2020, volume: 135, edition: 21, DOI
LYNX – modulární bioinformatický nástroj s uživatelským rozhraním pro analýzu NGS da u pacientů s hematologickými malignitami

REIGL Tomáš NAVRKALOVÁ Veronika HYNŠT Jakub PORC Jakub Paweł PÁL Karol HRON T. HALBHUBER Z. POSPÍŠILOVÁ Šárka DARZENTAS Nikos PLEVOVÁ Karla

Conference abstract

Year: 2020, type: Conference abstract
LYNX (Lymphoid NGS): a versatile NGS panel for comprehensive analysis of molecular markers with clinical relevance in lymphoproliferative disorders

NAVRKALOVÁ Veronika HYNŠT Jakub PLEVOVÁ Karla PÁL Karol REIGL Tomáš PORC Jakub Paweł DARZENTAS Nikos MAREČKOVÁ Andrea JELÍNKOVÁ Hana ŽENATOVÁ Marcela PAVLOVÁ Šárka KOTAŠKOVÁ Jana POSPÍŠILOVÁ Šárka

Conference abstract

Year: 2020, type: Conference abstract
Minor-clone TP53 mutations in CLL patients entering first-line treatment: clonal evolution and clinical impact.

MALČÍKOVÁ Jitka PAVLOVÁ Šárka VONKOVÁ Barbara KOTAŠKOVÁ Jana RADOVÁ Lenka PLEVOVÁ Karla ŽENATOVÁ Marcela HYNŠT Jakub DVOŘÁČKOVÁ Barbara PANOVSKÁ Anna BRYCHTOVÁ Yvona TICHÝ Boris TOM Nikola PÁL Karol MAYER Jiří DOUBEK Michael POSPÍŠILOVÁ Šárka

Conference abstract

Year: 2020, type: Conference abstract