Cholinesterase-based sensors for organophosphates and carbamates
P. Skládal. Determination of organophosphate and carbamate pesticides using a cobalt phthalocyanine-modified carbon paste electrode and a cholinesterase enzyme membrane. Anal. Chim. Acta 252, 11-5 (1991).
P. Skládal & M. Mascini. Sensitive detection of pesticides using amperometric sensors based on cobalt phthalocyanine-modified composite electrodes and immobilized cholinesterase. Biosens. Bioelectron. 7, 335-43 (1992).
P. Skládal. Detection of organophosphate and carbamate pesticides using disposable biosensors based on chemically modified electrodes and immobilized cholinesterase. Anal. Chim. Acta, 269, 281-7 (1992).
P. Skládal, M. Pavlík & M. Fiala. Pesticide biosensor based on coimmobilized acetylcholinesterase and butyrylcholinesterase. Anal. Lett. 27, 29-40 (1994).
P. Skládal, M. Fiala & J. Krejčí. Detection of pesticides in the environment using biosensors based on cholinesterases.
Int. J. Environ. Anal. Chem., 65, 139-48 (1996).P. Skládal & J. Krejčí. Performance of the amperometric biosensor with immobilis
ed butyrylcholinesterase in organic solvents. Collect. Czech. Chem. Commun., 61, 985-91 (1996).P. Skládal, G. S. Nunes, H. Yamanaka & M. L. Ribeiro. Detection of carbamate pesticides in vegetable samples using cholinesteras-based biosensors. Electroanalysis, 9, 1083-7 (1997).
Construction and application of enzyme electrodes
P. Skládal. Mushroom tyrosinase modified carbon paste electrode as an amperometric biosensor for phenols. Collect. Czech. Chem. Commun. 56, 1427-33 (1991).
L. Macho
lán, P. Skládal, I. Boháčková & J. Krejčí. Amperometric glucose biosensor with extended concentration range utilizing complexation effect of borate. Biosens. Bioelectron. 7, 593-8 (1992).P. Skládal, M. Mascini, C. Salvadori & G. Zannoni. Detection of bacterial contamination in milk using an L-lactate biosensor. Enzyme Microb. Technol. 15, 508-12 (1993).
P. Skládal. Effects disturbing signal stability of amperometric biosensors for long-term measurements. Bioelectrochem. Bioenerg. 32, 145-54 (1993).
M. Petř
ivalský, P. Skládal, L. Macholán & J. Volc. Amperometric enzyme electrodes for substrates of immobilized pyranose oxidase. Collect. Czech. Chem. Commun. 59, 1226-34 (1994).T. Kaláb & P. Skládal. Evaluation of mediators for development of amperometric microbial bioelectrodes. Electroanalysis 6, 1004-8. (1994).
P. Skládal. Compensation of temperature variations disturbing performance of an amperometric biosensor for continuous monitoring. Sens. Actuators B, 28, 59-62 (1995).
Electrochemical immunosensors for pesticides
T. Kaláb & P. Skládal. A disposable amperometric immunosensor for 2,4-dichlorophenoxyacetic acid. Anal. Chim. Acta, 304, 361-8 (1995).
P. Skládal & T. Kaláb. A multichannel immunosensor for 2,4-dichlorophenoxyacetic acid. Anal. Chim. Acta, 316, 73-8 (1995).
T. Kaláb & P. Skládal. Disposable multichannel immunosensors for 2,4-dichlorophenoxyacetic acid using acetylcholinesterase as an enzyme label. Electroanalysis, 9, 293-7 (1997).
Piezoelectric biosensors and characterization of affinity interactions
M. Minunni, P. Skládal & M. Mascini. A piezoelectric quartz crystal as a direct affinity sensor. Anal. Let. 27, 1475-87 (1994).
P. Skládal, M. Minunni, M. Mascini, V. Kolář & M. Fránek. Characterization of monoclonal
antibodies to 2,4-dichlorophenoxyacetic acid using a piezoelectric quartz crystal microbalance in solution. J. Immunol. Meth. 176, 117-25 (1994).M. Minunni, P. Skládal & M. Mascini. A piezoelectric crystal biosensor for detection of atrazine. Life Chemistry Reports 10, 391-8 (1994).
C. Steegborn & P. Skládal. Construction and characterisation of the direct piezoelectric immunosensor for atrazine operating in solution. Biosens. Bioelectron., 12, 19-27 (1997).
J. Horáček & P. Sklá
dal. Development of direct piezoelectric biosensor operating in liquid solution for the competitive immunoassay of 2,4-dichlorophenoxyacetic acid. Anal. Chim. Acta, 347, 43-50. (1997).
P. Skládal, L. Macholán, M. Petřivalský, I. Pluháček & L. Stančík. Sensing of insecticides using amperometric enzyme biosensors.
GIT Fachzeitschr. Labor. 7 (1993) 606-7.P. Skládal. Piezoelektrické biosensory. Chem. Listy, 89, 170-9 (1995).
P. Skládal. Cholinesterase-based biosensors for detection of pesticides. Food Technol. Biotechnol., 34, 43-9 (1996).
P. Skládal. Použití biosensorů ke studiu afinitních interakcí biomolekul.
Chem. Listy, 90, 863-72 (1996).P. Skládal & L. Macholán. Biosensory - současný stav a perspektivy. Chem. Listy, 91, 105-13 (1997).
P. Skládal. Advances in electrochemical immunosensors. Electroanalysis, 9, 737-45 (1997).
Course on biosensors - content
Definition of biosensors. Historic overview. Characteristics of ideal biosensors. Basic measuring procedures.
Electrochemical biosensors, enzyme electrodes. Potentiometric systems. Reference electrodes. Amperometric measurement of oxygen, hydrogen peroxide and NADH, mediators for the transfer of electrons from biomolecules to electrodes. Composite and organometallic electrodes. Conductimetric systems, measurement of impedance, voltammetry.
Optical systems. Spectrophotometric, fluorimetric and luminescence-based biosensors, use of optical fibres. Characterisation of the affinity interactions of biomolecules using ewanescent waves - surface plasmon resonance, integrated optical couplers and related techniques.
Piezoelectric systems, calorimetric biosensors, use of field effect transistors.
Biochemical (biological) components of biosensors. Enzyme-based biocatalytic sensors, determination of inhibitors and enzyme activities. Microbial, tissue and receptor-based biosensors.
Bioaffinity systems. Immunosensors. Nucleic acids as biorecognition elements.
Technology of biosensors. Immobilisation of biomolecules, activation of inorganic supports, methods for covalent binding, membrane techniques, electropolymerisation. Miniaturisation and mass production, screen printing, thick- and thin-film sensors. Biosensors as a part of integrated circuits.
Enzyme reactors, microdialysis, flow systems and flow injection analysis combined with biosensors. Performance of biosensors in organic solvents. Elimination of the effects of interferences. Systems for biochemical amplification.
Basic theories of the function of biosensors. Mass transport, mathematic models, prediction of experimental parameters.
Applications of biosensors. Clinical laboratory, determination of metabolites in vivo. Environmental monitoring of toxic compounds. Food and pharmaceutic industries, control of processes in biotechnology. Commercially available biosensors.