This course offers information about plant cell, tissue and organ culture in vitro. Basic methods cover in vitro vegetative multiplication of herbs and woody plants, callus culture, somatic embryogenesis, test-tube pollination/fertilization, embryo culture and their availability for plant breeding. Genetic manipulation in vitro is represented by methods of androgenesis (anther culture, microspore and pollen culture), gynogenesis (ovary and ovule culture) and dihaploidization. Direct and indirect methods of plant genome transformation are mentioned as well. Special attention is devoted to plant hormones and growth regulators used for initiation and maintenance of in vitro cultures. This course is taught in every spring semester. There are 2 hrs of lectures and 2 hrs of practice scheduled for every week.