Medium 96: MINERAL MEDIUM WITH VITAMINS

    Na2HPO4                     2.44 g
    KH2PO4                      1.52 g
    (NH4)2SO4                    0.5 g
    MgSO4.7H2O                   0.2 g
    CaCl2.2H2O                  0.05 g
    Trace element solution*       10 ml
    Vitamin solution**           2.5 ml
    Distilled water             1000 ml
    pH 6.9
    Sterilize at 115°C for 15 min.

    *Trace element solution
    EDTA                         0.5 g
    FeSO4.7H2O                   0.2 g
    Trace element solution (see
    medium 65)                   100 ml
    Distilled water              900 ml

    **Vitamin solution
    p-Aminobenzoate                1 mg
    Biotin                       0.2 mg
    Nicotine acid                  2 mg
    Thiamine                       1 mg
    Ca-Pantothenate              0.5 mg
    Pyridoxamine                   5 mg
    Cyanocobalamine                2 mg
    Distilled water              100 ml