Peptone 10 g Lab Lemco (Oxoid) 8 g Yeast extract 3 g Glucose 5 g Lactose 5 g KH2PO4 2.5 g K2HPO4 2.5 g MgSO4.7H2O 0.2 g MnSO4.4H2O 0.05 g Distilled water 1000 ml pH 6.8 Sterilize at 115 °C for 20 min.