Yeast extract 5 g Glucose 20 g (NH4)2SO4 3 g KH2PO4 1 g K2HPO4.3H2O 0.16 g MgSO4.7H2O 0.7 g NaCl 0.5 g Ca(NO3)2.4H2O 0.4 g Agar 20 g Distilled water 1000 ml pH 4.0