I. Yeast extract 3 g Peptone 5 g NaCl 50 g MnSO4 0.05 g Agar 25 g Distilled water 800 ml Sterilize at 121°C for 15 min. II. Na2CO3 10 g Distilled water 200 ml Sterilize through Seitz filter. Mix together I. and II. and distribute the medium into sterile tubes or Petri dishes. pH not adjusted (=9.5).