Yeast extract 5 g Beef extract 5 g Tryptose 10 g Proteose peptone No.3 10 g NaCl 5 g Agar 20 g Distilled water 1000 ml pH 7.2 Sterilize at 121°C for 15 min., cool to 50°C and add sterile L-Cysteine.HCl (10%) 2.5 ml Sheep blood 100 ml