Yeast extract 5 g Beef extract 2 g Tryptone 10 g Glucose 2 g NaCl 5 g L-Cysteine.HCl 0.3 g Agar 20 g Distilled water 1000 ml Adjust to pH 7.4. Sterilize at 115°C for 20 min., cool to 50°C and add 100 ml sterile sheep or horse blood.