Peptone 10 g
Casein peptone, tryptic digest 10 g
Tomato juice* 400 ml
Agar 20 g
Distilled water 600 ml
Dissolve the solids in water by heating. Add
tomato juice, mix, and sterilize at 121°C for 20
min. The final pH value of the medium should be
6.0 - 6.2.
*Tomato juice:
Tomatoes 250 g
Distilled water 500 ml
Cut up tomatoes and steam in water for an hour or
until they are pulped. Clarify through gauze and
filter through paper.