I. Yeast extract 10 g
Casamino acids 7.5 g
Sodium citrate 3 g
KCl 2 g
MgSO4.7H2O 20 g
FeCl2 traces
NaCl 250 g
Distilled water 750 ml
II. Agar 25 g
Distilled water 250 ml
Steam separately the two solutions, mix together
and adjust pH to 7.4. Sterilize at 121°C for 15
min. and distribute the medium into sterile tubes
or Petri dishes.