Medium 44: ALICYCLOBACILLUS ACIDOCALDARIUS MEDIUM

    I. Yeast extract               1 g 
    Glucose                        1 g 
    (NH4)2SO4                    0.2 g 
    KH2PO4                         3 g 
    MgSO4.7H2O                   0.5 g 
    CaCl2.2H2O                  0.25 g 
    Distilled water              500 ml 
    pH 3.0 - 4.0. 
    Sterilize at 121°C for 10 min. 

    II. Agar                      30 g 
    Distilled water              500 ml 
    Sterilize at 121°C for 20 min. 
 
Sterilize separately solutions I. and II. and cool
 to 50°C. Thoroughly mix the medium before 
distributing aseptically into sterile tubes or 
Petri dishes.