L-Asparagine (anhydrous)* 1 g
Glycerol 10 g
K2HPO4 1 g
Trace element solution** 1 ml
The pH of this solution is about 7.0 - 7.4. Do not
adjust if it is within this range.
Agar 20 g
Distilled water 1000 ml
*Grind asparagine and dilute in a small volume of
distilled water acidified with HCl.
**Trace element solution:
FeSO4.7H2O 0.1 g
MnCl2.4H2O 0.1 g
ZnSO4.7H2O 0.1 g
Distilled water 100 ml