Yeast extract 1.0 g Proteose peptone (Difco No.3) 1.0 g Casamino acids (Difco) 1.0 g Glucose 1.0 g NaCl 1.0 g Na pyruvate 0.5 g MgSO4 x 7 H2O 0.1 g K2HPO4 0.6 g Agar 15.0 g Distilled water 1000.0 ml Adjust the pH to 7.2. Sterilize at 121 °C for 15 min.