Peptone 5 g Yeast extract 1 g Fe(III) citrate 0.1 g NaCl 19.4 g MgCl2 8.8 g Na2SO4 3.24 g CaCl2 1.8 g KCl 0.55 g NaHCO3 0.16 g KBr 0.08 g SrCl2 34 mg H3BO3 22 mg Na-silicate 4 mg NaF 2.4 mg (NH4)NO3 1.6 mg Na2HPO4 8 mg Distilled water 1000 ml Adjust pH to 7.6 +/- 0.2 at 25 °C. Sterilize at 121°C for 15 min. Add 15 g agar for solid media. Recommended medium is supplied by Carl Roth GmbH + Co.