Peptone 5 g Meat Extract (Lab-Lemco) 5 g Tryptone 10 g Yeast Extract 5 g Glucose 10 g Tween 80 1 ml K2HPO4.3H2O 2 g Sodium acetate anhydrous 5 g Diammonium hydrogen citrate 2 g MnSO4.H2O 0.25 g MgSO4.7H2O 0.1 g Agar 15 g Distilled water 1000 ml Sterilize at 118°C for 20 min. pH 6.2-6.4