Yeast extract 0.05 g Protease Peptone No. 3 0.05 g Casamino Acids 0.05 g Dextrose or glucose 0.05 g Soluble starch 0.05 g Sodium pyruvate 0.03 g K2HPO4 0.03 g MgSO4.7H2O 0.005 g Distilled water 16.67 ml SW30 83.33 ml (bacteriological agar 2 g) Adjust pH to 7.5 Sea Water Solution 30% (SW30) NaCl 234 g MgCl.6H2O 39 g MgSO4.7H2O 61 g *CaCl2 1 g KCl 6 g *NaHCO3 0.2 g NaBr 0.7 g Distilled water 1000 ml