Glucose 40 g Pepton 10 g Yeast extract 10 g Citric acid 1.37 g Na2HPO4.2H2O 3.38 g Agar 15 g Distilled water 980 ml Sterilized at 121°C for 20 min. Add 10 ml of glacial acetic acid and 10 ml of absolute ethanol (sterilized using filtration) Final pH ca. 3.8 (pH is not adjusted)