Medium 163: ISP 2 AGAR WITH ARTIFICIAL SEAWATER

   Yeast extract                4 g
   Malt extract                10 g
   Glocose                      4 g
   Agar                        18 g
   Artificial seawater       1000 ml          	

Adjust pH to 7.2 +/-0.2.
Sterilized at 118 °C for 20 min.

   Artificial seawater:
   NaCl	                      234.7 g
   Na2SO4                      39.2 g
   MgCl2.6H2O                 106.4 g
   CaCl2                         11 g
   NaHCO3                      1.92 g
   KCl	                       6.64 g
   KBr	                       0.96 g
   H3BO3                       0.26 g
   Distilled water           10 000 ml