NaCl 78 g MgCl2.6H2O 13 g MgSO4.7H2O 20.3 g KCl 2 g NaBr 0.23 g NaHCO3 0.06 g CaCl2.2H2O 0.33 g Yeast extract 5 g Distilled water 1000 ml Adjust pH to 7.2 - 7.4. Sterilized at 121°C for 15 min.