Yeast extract 4 g Glucose 4 g Malt extract 5 g Agar 18 g Destilled water 1000 ml Salt solution: FeSO4.7H2O 0.2 g MnCl2.7H2O 0.1 g ZnSO4.7H2O 0.1 g Destilled water 100 ml pH 7.2 Sterilize at 115°C for 15 min. Vitamin solution: Pyridoxin 0.5 mg Biotin 0.5 mg Distilled water 1 ml Sterilize separately by filtration.