Medium 12: SPHAEROTILUS - LEPTOTHRIX MEDIUM

    Yeast extract                  1 g 
    Peptone                      1.5 g 
    MgSO4.7H2O                   0.2 g 
    CaCl.2H20                   0.05 g 
    Ferric ammonium citrate      0.5 g 
    MnSO4.H2O                   0.05 g 
    FeCl3.6H2O                  0.01 g 
    Agar                          20 g 
    Tap water                   1000 ml 
    pH 7.1