Trypton 10 g
NaCl 5 g
HEPES-buffer
(Fluka No.54461) 11.9 g
Glucose 2 g
Agar 30 g
Distilled water 1000 ml
pH 7.0
Sterilize at 115°C for 20 min., cool to 50°C and
add sterile 60 mg ampicillin/1L.