Medium 114: VOGEL-BONNER MEDIUM
Salt solution* 2 ml
Dextrose (Difco) solution** 6.7 ml
L-Histidine.HCl solution*** 1 ml
Biotin solution**** 0.6 ml
Agar (Difco B140) 2 g
Deionized water (sterile) 88.7 ml
*Salt solution
MgSO4.7H2O 1 g
Citric acid (monohydrate) 10 g
K2HPO4 50 g
Na(NH4)HPO4.4H2O 17.5 g
Dissolve and complete to 100 ml with deionized
water.
**30% dextrose (Difco B155) solution
***L-Histidine solution
L-Histidine.HCl 500 mg
Redistilled water 100 ml
****Biotin solution
Biotin 12.36 mg
Redistilled water 100 ml
l. Ampicillin solution
Ampicillin 80 mg
NaOH (0.02 N) 10 ml
2. Tetracycline solution
Tetracycline 80 mg
HCl (0.02 N) 10 ml
Dissolve agar in deionized water and sterilize at
121°C for 15 min. Cool to 50°C and add
filter-sterilized solutions *, **, ***, ****.
Futher add 0.315 ml filter-sterilized ampicillin
solution (1) for strains CCM 3808-CCM 3812 and
0.315 ml ampicillin (1) and 0.025 ml tetracycline
solutions (2) for CCM 3813.