Glucose 0.15 g
(NH4)2SO4 0.5 g
CaCO3 0.1 g
Ca(NO3)2 20.1 g
KCl 0.05 g
K2HPO4 0.05 g
MgSO4.7H2O 0.05 g
Na2S.9H2O 0.187 g
Vitamin solution* 1 ml
Distilled water 1000 ml
Sterilize separately basic medium and add
aseptically sterile vitamin solution.
*Vitamin solution:
Calcium pantothenate 1 mg
Niacin 1 mg
Biotin 0.005 mg
Cyanocobalamin 0.005 mg
Folic acid 0.005 mg
Pyridoxine 1 mg
p-Aminobenzoic acid 1 mg
Cocarboxylase 1 mg
Inositol 1 mg
Thiamine 1 mg
Riboflavin 1 mg