Medium 109: ALICYCLOBACILLUS MEDIUM

    I. Yeast extract               6 g
    Glucose                        5 g
    CaCl2.2H2O                  0.25 g
    MgSO4.7H2O                   0.5 g
    (NH4)2SO4                    0.2 g
    KH2PO4                         3 g
    Distilled water              500 ml
    pH 4.0
    Sterilize at 115°C for 15 min.

    II. Trace element solution*    1 ml

    III. Agar                     30 g
    Distilled water              500 ml
    Sterilize at 121°C for 15 min.

    *Trace element solution:
    ZnSO4.7H2O                   0.1 g
    MnCl2.4H2O                  0.03 g
    H3BO3                        0.3 g
    CuCl2.6H2O                   0.2 g
    CaCl2.2H2O                  0.01 g
    NiCl2.6H2O                  0.02 g
    Na2MoO4.2H2O                0.03 g
    Distilled water             1000 ml
    Sterilize at 121°C for 15 min.

Mix together I - III and distribute the medium 
aseptically into sterile tubes or Petri dishes.