Effects of controlled oxidative stress and uncouplers on primary photosynthetic processes in vegetative cells of Antarctic alga Zygnema sp.

Christos Kakkou, Miloš Barták, Josef Hájek, Kateřina Skácelová, Jana Hazdrová

10.5817/CPR2016-1-10

In our study, we present responses of Antarctic strain of filamentous alga Zygnema sp. collected at James Ross Island (Antarctica) to application of variuos uncouplers of pri-mary photosynthetic processes. We exposed the alga to different concentrations of nigericin, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), dithiothreitol (DTT), methyl viologen (MV) and hydrogen peroxide (H₂O₂) in order to test stability of photosystem II, involvement of non-photochemical quenching, and PS II functioning under combination of moderate light with particular uncoupler. Oxidative stress tolerance was tested by the combination of hydrogen peroxide (H₂O₂) and moderate light. Time courses of F_V/F_M, F_PSII, NPQ and qF₀ were investigated and particular effects of the above-specified chem-icals discussed. Moderate doses of uncouplers allowing partial recovery, and the doses causing full inhibition of PS II were specified.       
 

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